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Positional Oligomer Importance Matrices

POIMs: a way of extracting discriminative features from the descision function of SVMs by taking into account the correlation structure of these features.

At the heart of many important bioinformatics problems, such as gene finding and function prediction, is the classification of biological sequences. Frequently the most accurate classifiers are obtained by training support vector machines (SVMs) with complex sequence kernels. However, a cumbersome shortcoming of SVMs is that their learned decision rules are very hard to understand for humans and cannot easily be related to biological facts.

To make SVM-based sequence classifiers more accessible and profitable, we introduce the concept of Positional Oligomer Importance Matrices (POIMs) and propose an efficient algorithm for their computation. In contrast to the raw SVM feature weighting, POIMs take the underlying correlation structure of k-mer features induced by overlaps of related k-mers into account. POIMs can be seen as a powerful generalization of sequence logos: they allow to capture and visualize sequence patterns that are relevant for the investigated biological phenomena.

We are currently working on methods to visualize and condense the information extracted via POIMs in order to find discriminative motifs for different signals on the DNA like splice sites, promoters or polyadenylation sites.

Downloadable Material

The paper can be downloaded here (local) and here (served by OUP Bioinformatics). Supplemental material for the manuscript "Positional Oligomer Importance Matrices -- Understanding Support Vector Machine Based Signal Detectors" by Sören Sonnenburg, Alexander Zien, Petra Philips and Gunnar Rätsch.

Online Tool

At our Galaxy site you can try POIMs with an uploaded set of sequences. First go to http://galaxy.fml.tuebingen.mpg.de and select "SVM Toolbox->Positional Oligomer Matrices" from the list of tools at the left. Then (i) upload two sets of sequences, (ii) train an SVM, and (iii) have the POIMs displayed!

Stand-a-lone Tool

This is the collection of scripts via which we generated the figures in the paper. This tool will require shogun to run. Grab it here.

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